Journal: Bioscience Reports

Article Title: Hydrogen sulfide attenuates sepsis-induced cardiac dysfunction in infant rats by inhibiting the expression of cold-inducible RNA-binding protein

doi: 10.1042/BSR20241398

Figure Lengend Snippet: ( A–F ) Representative Western blots and quantitative analysis for GRP78, ATF6, p-PERK, PERK, p-IRE1-a, IRE1-a. GAPDH was used as the internal control. ( G ) Representative H&E staining left ventricular sections (scale bar = 50μm μm). ( H ) Severity scores of heart tissue sections. Data are mean ± SD from four independent experiments ( n = 6). One-way analysis of variance followed by LSD test with equal variance, and the Dunnett’s T3 method was performed if there was missing variance, P ＜0.05 was considered significant vs. sham + V. H&E, hematoxylin and eosin.

Article Snippet: The membranes were blocked with 3% BSA for 1.5 h at room temperature, and antigens were detected using the following antibodies at 4°C overnight: GRP78 (1:10000, Santa Cruz Biotechnology Inc., TX, U.S.A., sc-166490), ATF6 (1:500, MCE, SH, China, HY- P80379 ), p-PERK (1:1000, GeneTex, SZ, China, GTX00673), PERK (1:1000, Santa Cruz Biotechnology, TX, U.S.A., sc-377400), p-IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab48187), IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab37073), CIRP (1:5000, Proteintech Biotechnology, WH, China, 10209-2-AP), CSE (1:1000, Proteintech Biotechnology, WH, China, 60234-1-Ig), and GAPDH (1:5000, Proteintech Biotechnology, WH, China, 10494-1-AP).

Techniques: Western Blot, Control, Staining

Journal: Bioscience Reports

Article Title: Hydrogen sulfide attenuates sepsis-induced cardiac dysfunction in infant rats by inhibiting the expression of cold-inducible RNA-binding protein

doi: 10.1042/BSR20241398

Figure Lengend Snippet: ( A−F ) Representative Western blots and quantitative analysis for GRP78, ATF6, p-PERK, PERK, p-IRE1-a, IRE1-a. GAPDH was used as the internal control. ( G ) Representative H&E staining left ventricular sections (scale bar = 50μm μm). ( H ) Severity scores of heart tissue sections. Data are mean ± SD from four independent experiments ( n = 6). One-way analysis of variance followed by LSD test with equal variance, and the Dunnett’s T3 method was performed if there was missing variance, P ＜0.05 was considered significant vs. sham + V. H&E, hematoxylin and eosin.

Article Snippet: The membranes were blocked with 3% BSA for 1.5 h at room temperature, and antigens were detected using the following antibodies at 4°C overnight: GRP78 (1:10000, Santa Cruz Biotechnology Inc., TX, U.S.A., sc-166490), ATF6 (1:500, MCE, SH, China, HY- P80379 ), p-PERK (1:1000, GeneTex, SZ, China, GTX00673), PERK (1:1000, Santa Cruz Biotechnology, TX, U.S.A., sc-377400), p-IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab48187), IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab37073), CIRP (1:5000, Proteintech Biotechnology, WH, China, 10209-2-AP), CSE (1:1000, Proteintech Biotechnology, WH, China, 60234-1-Ig), and GAPDH (1:5000, Proteintech Biotechnology, WH, China, 10494-1-AP).

Techniques: Western Blot, Control, Staining

Journal: Bioscience Reports

Article Title: Hydrogen sulfide attenuates sepsis-induced cardiac dysfunction in infant rats by inhibiting the expression of cold-inducible RNA-binding protein

doi: 10.1042/BSR20241398

Figure Lengend Snippet: CIRP and marker proteins amount of endoplasmic reticulum stress was significantly decreased after NaHS treatment:( A-–G ) Representative Western blots and quantitative analysis for CIRP, GRP78, ATF6, p-PERK, PERK, p-IRE1-a, and IRE1-a. GAPDH was used as the internal control. ( H ) Representative H&E staining left ventricular sections (scale bar = 50 μm). ( I ) Severity scores of heart tissue sections. Data are mean ± SD from four independent experiments ( n = 6). One-way analysis of variance followed by LSD test with equal variance, and the Dunnett’s T3 method was performed if there was missing variance, P ＜0.05 was considered significant vs. Ssham + V. H&E, hematoxylin and eosin.

Article Snippet: The membranes were blocked with 3% BSA for 1.5 h at room temperature, and antigens were detected using the following antibodies at 4°C overnight: GRP78 (1:10000, Santa Cruz Biotechnology Inc., TX, U.S.A., sc-166490), ATF6 (1:500, MCE, SH, China, HY- P80379 ), p-PERK (1:1000, GeneTex, SZ, China, GTX00673), PERK (1:1000, Santa Cruz Biotechnology, TX, U.S.A., sc-377400), p-IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab48187), IRE1-a (1:1000, Abcam; Cambridge, MA, U.S.A., ab37073), CIRP (1:5000, Proteintech Biotechnology, WH, China, 10209-2-AP), CSE (1:1000, Proteintech Biotechnology, WH, China, 60234-1-Ig), and GAPDH (1:5000, Proteintech Biotechnology, WH, China, 10494-1-AP).

Techniques: Marker, Western Blot, Control, Staining